理論と実験の協奏による柔らかな分子系の機能の科学-文部科学省科学研究費補助金「新学術領域研究」領域代表者 田原太平 (平成25-29年度) やわぶん

   

28th Workshop

28th Workshop
Bacterial Flagella: From Functions of Soft Biomolecular Assemblies to Cell Motility
【 Date 】 March 11-13, 2018
【 Venue 】 Biwako Grand Hotel, Kyō-ōmi
【 Access 】 http://www.biwakogh.co.jp/en/access.html

Program: March 11, 2018

12:30 Registration
13:20 Welcome & Announcement
Akihiko Ishijima (Osaka Univ.)
Export & Assembly
Discussion Leader: Naoya Terahara (Osaka Univ.)
13:30-13:45 Metal ion binding of the Methanococcus archaellin required for filament integrity
Satoshi Shibata (OIST)
13:45-14:00 Characterization of flagellins isolated from Lactobacillus agilis
Akinobu Kajikawa (Tokyo Univ. Agri.)
14:00-14:15 Initiation of the polar flagellar assembly facilitated by FlhF, the positive regulator for flagellar number in Vibrio alginolyticus
Yuna Inoue (Nagoya Univ.)
14:15-14:30 Biochemical analysis of FlhG, ATPase Involved in regulating the number of flagella of Vibrio alginolyticus
Yoshino Imura (Nagoya Univ.)
14:30-14:40 Coffee-Tea break (10min)
Discussion Leader: Takayuki Kato (Osaka Univ.)
14:40-14:55 The zoospores of Actinoplanes missouriensis adhere to a solid surface by the type 4 pili
Tomohiro Kimura (Univ. Tokyo)
14:55-15:10 The biological function of SsaH as a chaperone in SPI2-T3SS
Akiko Takaya (Chiba Univ.)
15:10-15:25 Detection of the flagellar type III protein export by fluorescence
Keiya Takata (Osaka Univ.)
15:25-15:50 Toward a more complete understanding of the bacterial flagellar growth
Chien-Jung Lo* (National Central University)
15:50-16:00 Coffee-Tea break (10min)
Discussion Leader:Tohru Minamino (Osaka Univ.)
16:00-16:15 The flagellar secretrion signal - a new approach
Dara Niketic (University of Utah)
16:15-17:00 The latest from the Hughes lab
Kelly Hughes** (University of Utah)
17:00-17:30 Break and Check-in (30min)
17:30-19:00 Training course (90min)
Akihiko Isijima (Osaka Univ.)、Hajime Fukuoka (Osaka Univ.)
19:15-21:15 Dinner
21:15- Discussion
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Program: March 12, 2018

Breakfast
Rotor & Stator
Discussion Leader: Shuichi Nakamura (Tohoku Univ.)
9:00-9:25 Structure analysis of the flagellar basal body by electron cryomicroscopy.
Tomoko Miyata* (Osaka Univ.)
9:25-9:40 Eluciation of the ring formation mechanism of MS ring which is the center of bacterial flagellar motor formation
Keiichi Hirano (Nagoya Univ.)
9:40-9:55 Ring structure of FliL, a flagellar stator associated protein
Norihiro Takekawa (Osaka Univ.)
9:55-10:10 FliL roles on the two sets of Bradyrhizobium diazoefficiens flagellar systems
Florencia Menguccia (Nagoya Univ.)
10:10-10:25 Coffee-Tea break (15min)
Discussion Leader: Hiroyuki Terashima (Nagoya Univ.)
10:25-10:40 Elucidation of the ion selectivity mechanism of the Na+, K+-driven flagellar stator from Alkaliphilic Bacillus trypoxylicola
Syun Naganawa (Toyo Univ.)
10:40-10:55 Detection of structural of marine Vibrio bacterium flagellar stator PomA cytoplasmic loop
Taira Mino (Nagoya Univ.)
10:55-11:10 Role of Threonine residues in ion permeation for the Na+ driven flagellar motor PomA/PomB: insights from MD simulations
Ai Shinobu (Tokyo Tech.)
11:10-11:25 Structure of a periplasmic fragment of MotS from Bacillus alcalophilus
Koki Nishiuchi (Osaka Univ.)
11:25-11:40 Directed evolution of motAB using in vivo mutagenesis
Tatsuya Morishita (NICT )
11:40-11:55 Group Photo
11:55-13:00 Lunch
Chemotaxis
Discussion Leader: Norihiro Talekawa (Osaka Univ.)
13:00-13:15 Temperature-dependent control of a taurine chemoreceptor Mlp37 of Vibrio cholerae
So-ichiro Nishiyama (Hosei Univ.)
13:15-13:30 Distinct mechanisms of ligand recognition between Mlp24 and Mlp37, chemoreceptor proteins of Vibrio cholera
Yohei Takahashi (Osaka Univ.)
13:30-13:45 Rotation control of the single polar fagellum of Vibrio cholerae
Yuki Miura (Hosei Univ.)
13:45-14:00 Transducers and soluble receptors for chemotaxis of Vibrio alginolyticus
Mariko Momma (Hosei Univ.)
14:00-14:15 Coffee-Tea break (15min)
Discussion Leader: Yusuke Morimoto (Kyutech)
14:15-14:30 What is the Kapsel?
Shin-Ichi Aizawa (PUH)
14:30-14:45 Relationship between chemotactic response and size of E. coli.
Hiroto Tanaka (NICT)
14:45-15:00 Pressure-Induced CheY-FliM Dissociation Simulated by Parallel Cascade Selection Molecular Dynamics
Hiroaki Hata (Tokyo Tech.)
15:00-15:25 Single cell measurement for the chemotaxis proteins and cellular behavior
Hajime Fukuoka* (Osaka Univ.)
15:25-15:40 Technicologies
Nicon.
15:40-15:55 Coffee-Tea break (15min)
Discussion Leader: Seiji Kojima (Nagoya Univ.)
15:55-16:10 Swimming in complex environments
Jerko Rosko (University of Edinburgh)
16:10-16:55 Transmembrane Signaling Mechanisms of E. coli Chemoreceptors
John (Sandy) Parkinson (University of Utah)
16:55-17:10 Coffee-Tea break (15min)
17:10-19:00 Training course (90min)
Akihiko Ishijima (Osaka Univ.)、Hajime Fukuoka (Osaka Univ.)
19:15-21:15 Dinner
21:15- Discussion
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Program: March 13, 2018

Breakfast
Flagellar Filament
Discussion Leader: Satoshi Sibata (OIST)
9:00-9:25 Visualizing flagellar motions of magnetotactic bacterium MO-1
Masayoshi Nishiyama* (Kyoto Univ.)
9:25-9:40 High resolution structural analysis of the flagellar hook of Salmonella enterica serovar Typhimurium
Peter Horvath (Osaka Univ.)
9:40-9:55 Structure of FimA, a major component protein of fimbriae of Porphyromonas gingivalis
Kodai Okada (Osaka Univ.)
9:55-10:10 The cap structure of the bacterial flagellar filament
Fumiaki Makino (Osaka Univ.)
10:10-10:25 Structure of the flagellar distal rod constructed by full-length FlgG
Saijo Yumiko (Osaka Univ.)
10:25-10:35 Coffee-Tea break (10min)
Flagellar Motor
Discussion Leader: Takashi Sagawa (NICT)
10:35-10:50 Functional analysis of a FliF-G deletion-fusion mutant flagellar motor
Tomofumi Sakai (Osaka Univ.)
10:50-11:05 The effect of MotB(D32E) on the stall torque of Escherichia coli flagellar motor
Taishi Kasai (Hosei Univ.)
11:05-11:20 ffect of temperature on assembly of the flagellar motor stator
Yuya Suzuki (Tohoku Univ.)
11:20-11:30 Coffee-Tea break (10min)
Discussion Leader: Masayoshi Nishiyama (Kyoto Univ.)
11:30-11:45 Measurement of flagellar motor rotation at low load
Tsubasa Ishida (Hosei Univ.)
11:45-12:00 Rotation assay of the flagellar motor under near zero load
Yuta Hanaizumi (Tohoku Univ.)
12:00-12:15 3D torque measurements of the archaeal motor
Seiji Iwata (Gakushuin Univ.)
12:15-12:30 Step analysis of Bacillus flagellar motor
Naoya Terahara (Osaka Univ.)
12:30 Conference Closing
Akihiko Ishijima (Osaka Univ.)
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